The Human Cells, Tissues, and Organoids (HCTO) Core strives to make research using human pluripotent stem cells and their derivatives accessible to all researchers.
Contact the Core at kuochan.weng@wustl.edu
Services Offered
Tissue Culture Facilities for Rent
Access to biosafety cabinets, a clean bench, incubators, microscopes, and all other equipment necessary for the culture of human pluripotent stem cells under a Core ESCRO protocol
Group and Individual Training
Multiple small group, hands-on classes are offered every year on the culture of human pluripotent stem cells, and their differentiation into various lineages (e.g. neural differentiation). Custom individual training is also available.
Human Embryonic Stem Cell Lines and Differentiated Cells
NIH-approved hESC lines (H1, H9), as well as differentiated cell types (e.g. neural progenitors, cardiomyocytes, astrocytes, etc)
Custom Workflows
Contact the Core directly to inquire about custom experimental workflows and differentiation schemes
IRB and ESCRO protocols
Assistance preparing IRB and ESCRO protocols
Pricing
Services
| Human Cells, Tissue, and Organoids | Price | Unit | Estimated Time | Notes |
|---|---|---|---|---|
| Human ESC* | *ESCRO protocol required | |||
| WA01 (H1) (WiCell) | $35 | Cryovial | In-stock | WiCell License fee at cost |
| WA09 (H9) (WiCell) | $35 | Cryovial | In-stock | WiCell License fee at cost |
| HUES9 (Harvard ES Bank) | $35 | Cryovial | In-stock | |
| Healthy Control iPSC* | *MTA may be needed | |||
| iPSC – AN1.1 (WashU Line) | $35 | Cryovial | In-stock | |
| iPSC – WTC11 (Coriel GM25256) | $35 | Cryovial | In-stock | |
| iPSC – WTC11-GCaMP6 (Gladstone) | $35 | Cryovial | In-stock | |
| iPSC – SCTi003A (STEMCELL Technologies) | $35 | Cryovial | In-stock | |
| iPSC – live culture | Request | 6-well plate | 2 weeks | |
| iPSC – expansion and cryopreserve service | $300 | 4-wells + 5 vials | 2 weeks | |
| iPSC-Derived Cells | ||||
| Neural Progenitors | Request | 3M / vial | In-stock | |
| Cardiomyocytes | $450 | 12-well plate (10M cells) | 4 weeks | |
| Retinal Pigment Epithelium | Request | T150 (100M cells) | 12 weeks | |
| Mesechymal Stem Cells | $1,070 | 12-well plate | 4 weeks | Material included |
| Adipocytes | $1,750 | 12-well plate | 8 weeks | Material included |
| iPSC-Derived Organoids | ||||
| Cerebral Brain Organoids | Request | Living Culture | 6 weeks | |
| Intestinal Organoids | Request | Cryovial | In-stock | |
| Lung Organoids | Request | TransWell | 6 weeks | |
| Common Cell Lines | ||||
| Normal Human Dermal Fibroblast | $35 | Cryovial | In-stock | |
| Normal Human Ventricular Fibroblast | $35 | Cryovial | In-stock | |
| HUVEC | $35 | Cryovial | In-stock | |
| Equipment | Price | |||
| Hood Rental | $30 / hour | Basic supply included | ||
| Flowcytometry (Beckman CytoFLEX) | Request | |||
| Service | Price | |||
| Staff-run Experiment | $80 / hour | Basic supplies included | ||
| Consultation | $70 / hour | |||
| Collaboration | % Effort |
Training
| Training | Price | Estimated Time | Notes |
|---|---|---|---|
| Group Training: comprehensive hPSC Culture | $550 | 3 half-days | April & November |
| Individual Training: customized hPSC Culture | $350 | 3 to 4 half-days | Upon request |
| Individual Training: cardiac differentiation | $250 | 2 weeks | Upon request |
| Custom Individual Training | $80 / hour | Upon Request |
Prices effective September 1, 2023 and are subject to change.
Personnel
Thorold Theunissen, PhD
Assistant Professor, Developmental Biology; co-Director Human Cells, Tissues, and Organoids Core
- Email: t.theunissen@wustl.edu
The Theunissen lab investigates the molecular mechanisms regulating distinct pluripotent stem cell states and their applications in regenerative medicine.
Lauren Boggs
Research Technician, Human Cells, Tissues, and Organoids Core
- Email: boggs@wustl.edu
Lauren joined the CRM HCTO in April 2022 as a research technician.