The Human Cells, Tissues, and Organoids (hCTO) Core provides knowledge and services relating to human pluripotent stem cells and their downstream lineages to researchers at WashU and beyond.

Products and Services:
- Human Pluripotent Stem Cell Lines: NIH-approved hESC lines and iPSC lines are available to WashU researchers (see table below).
- Cell Differentiation: Our staff can differentiate hESCs and iPSCs into downstream linages encompassing the endoderm (e.g. hepatocytes), mesoderm (e.g. cardiomyocytes, osteoblasts), and ectoderm (e.g. neurons, astrocytes).
- Tissue Culture Facilities for Rent: We provide access to biosafety cabinets, incubators, microscopes, and all other equipment necessary for the culture of human pluripotent stem cells under a Core ESCRO protocol.
- Group and Individual Training: Multiple small group, hands-on classes are offered every year on the culture of human pluripotent stem cells and their differentiation into various lineages (e.g. neural differentiation). Custom individual training is also available.
- Custom Workflows: Contact the hCTO Core directly to inquire about custom experimental workflows and differentiation schemes.
- IRB and ESCRO Protocols: We provide assistance preparing IRB and ESCRO protocols.
For questions, contact the hCTO Core Director, Dr. Laura Fischer, (laura.fischer@wustl.edu).
General Pricing
| Services | Hourly Rate |
|---|---|
| Staff Hours (bench/experiment work) | $85 |
| Consultation Hours (research, planning, meetings, and other dry lab work) | $70 |
| Cell Lines (WUSTL, affiliates only) |
|---|
| Human ESC (H1, H9, HUES-9) |
| Human iPSC (AN1.1, WTC-11, STCi003-A) |
| Established hESC or iPSC lines provided by the researcher |
| Equipment Rental |
|---|
| Biosafety Cabinet |
| CytoFlex S Flow Cytometer |
Prices effective October 1, 2025 and are subject to change.
Staff
Thorold Theunissen, PhD
Associate Professor, Developmental Biology; co-Director Human Cells, Tissues, and Organoids Core
The Theunissen lab investigates the molecular mechanisms regulating distinct pluripotent stem cell states and their applications in regenerative medicine.
Lauren Boggs
Research Technician II, Human Cells, Tissues, and Organoids Core
- Email: boggs@nospam.wustl.edu
Lauren joined the hCTO Core in April 2022 and has extensive experience in iPSC and hESC culture and differentiation.