The Human Cells, Tissues, and Organoids (HCTO) Core strives to make research using human pluripotent stem cells and their derivatives accessible to all researchers.

Contact the Core at kuochan.weng@wustl.edu

Services Offered

Tissue Culture Facilities for Rent

Access to biosafety cabinets, a clean bench, incubators, microscopes, and all other equipment necessary for the culture of human pluripotent stem cells under a Core ESCRO protocol

Group and Individual Training

Multiple small group, hands-on classes are offered every year on the culture of human pluripotent stem cells, and their differentiation into various lineages (e.g. neural differentiation). Custom individual training is also available.

Human Embryonic Stem Cell Lines and Differentiated Cells

NIH-approved hESC lines (H1, H9), as well as differentiated cell types (e.g. neural progenitors, cardiomyocytes, astrocytes, etc)

Custom Workflows

Contact the Core directly to inquire about custom experimental workflows and differentiation schemes

IRB and ESCRO protocols

Assistance preparing IRB and ESCRO protocols

Pricing

Services

Human Cells, Tissue, and OrganoidsPriceUnitEstimated TimeNotes
Human ESC**ESCRO protocol required
WA01 (H1) (WiCell)$35CryovialIn-stockWiCell License fee at cost
WA09 (H9) (WiCell)$35CryovialIn-stockWiCell License fee at cost
HUES9 (Harvard ES Bank)$35CryovialIn-stock
Healthy Control iPSC**MTA may be needed
iPSC – AN1.1 (WashU Line)$35CryovialIn-stock
iPSC – WTC11 (Coriel GM25256)$35CryovialIn-stock
iPSC – WTC11-GCaMP6 (Gladstone)$35CryovialIn-stock
iPSC – SCTi003A (STEMCELL Technologies)$35CryovialIn-stock
iPSC – live cultureRequest6-well plate2 weeks
iPSC – expansion and cryopreserve service$3004-wells + 5 vials2 weeks
iPSC-Derived Cells
Neural ProgenitorsRequest3M / vialIn-stock
Cardiomyocytes$45012-well plate (10M cells)4 weeks
Retinal Pigment EpitheliumRequestT150 (100M cells)12 weeks
Mesechymal Stem Cells$1,07012-well plate4 weeksMaterial included
Adipocytes$1,75012-well plate8 weeksMaterial included
iPSC-Derived Organoids
Cerebral Brain OrganoidsRequestLiving Culture6 weeks
Intestinal OrganoidsRequestCryovialIn-stock
Lung OrganoidsRequestTransWell6 weeks
Common Cell Lines
Normal Human Dermal Fibroblast$35CryovialIn-stock
Normal Human Ventricular Fibroblast$35CryovialIn-stock
HUVEC$35CryovialIn-stock
EquipmentPrice
Hood Rental$30 / hourBasic supply included
Flowcytometry (Beckman CytoFLEX)Request
ServicePrice
Staff-run Experiment$80 / hourBasic supplies included
Consultation$70 / hour
Collaboration% Effort
Non-WUSTL affiliates add 30%

Training

TrainingPriceEstimated TimeNotes
Group Training: comprehensive hPSC Culture$5503 half-daysApril & November
Individual Training: customized hPSC Culture$3503 to 4 half-daysUpon request
Individual Training: cardiac differentiation$2502 weeksUpon request
Custom Individual Training$80 / hourUpon Request
Non-WUSTL affiliates add 30%

Prices effective September 1, 2023 and are subject to change.

Personnel

Kuo-Chan Weng, PhD

Kuo-Chan Weng, PhD

Director, Human Cells, Tissues, and Organoids Core

Thorold Theunissen, PhD

Thorold Theunissen, PhD

Assistant Professor, Developmental Biology; co-Director Human Cells, Tissues, and Organoids Core

The Theunissen lab investigates the molecular mechanisms regulating distinct pluripotent stem cell states and their applications in regenerative medicine.

 

Theunissen Lab website »

Lauren Boggs

Lauren Boggs

Research Technician, Human Cells, Tissues, and Organoids Core

Lauren joined the CRM HCTO in April 2022 as a research technician.